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CRTAP present in the sample is added and binds to antibodies coated on the wells
The concentration of Human aFP in the samples is then determined by comparing the OD of the samples to the standard curve
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TGFb1
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to MIP1g
Human PTH (Parathyroid Hormone) ELISA Kit Packaging:96 wells CRTAP present in the sampleThe Human (PTH) Parathyroid Hormone ELISA Kit measures Parathyroid Hormone in samples. The plate has been pre coated with Human PTH antibody. PTH present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human PTH Antibody is added and binds to PTH in the sample. Then Streptavidin HRP is added and binds to the Biotinylated PTH antibody. After incubation unbound Streptavidin HRP is washed away during a washing
